Figure 7.
Tetrachlorodibenzo-p-dioxin (TCDD) and CPF exposure in FRTL-5 regulates the expression of genes involved in prevention of apoptosis and in mitogen-activated protein kinase (MAPK) signalling. (A) Bax/Bcl2 transcripts ratio in FRTL-5 exposed to TCDD 10−9 M for 1 or 7 days or vehicle only (DMSO). RT-qPCR was performed as detailed in M&M and primers are listed in Table S1. (B) Western blotting analyses of MAPK activation in FRTL-5 treated with TCDD 10−9 M for 1 and 7 days, compared to untreated cells (Ctrl). pERK1/ERK2 was determined as well as the cellular content of ERK1/ERK2. α-Tubulin was used as loading control. (C) Bax and Bcl2 transcripts ratio in FRTL-5 exposed to CPF 10−9 M for 1 or seven days or vehicle only (DMSO). (D) Western blotting analyses of MAPK activation in FRTL-5 treated with CPF 10−9 M for 1 and 7 days, compared to untreated cells (Ctrl). pERK1/ERK2 was determined as well as the cellular content of ERK1/ERK2. α-Tubulin was used as loading control. RT-qPCR data are reported as fold change values calculated as ratio between average relative gene expression in treated and control cells. Data are reported as the average and standard deviation of Gapdh-normalized mRNA levels. The black bar signs the control level. Results are expressed as the mean ± standard deviation of three independent experiments. (Bax/Bcl2, CPF p-value = 0.035, p = 0.048).