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. 2019 Jan 19;18:11. doi: 10.1186/s12934-019-1059-3

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Total protein analysis from culture supernatant. a SDS-PAGE 12% of protein obtained from baffled shake flask cultures, b SDS-PAGE 12% of protein obtained from bioreactor supernatants. Black arrows indicate the band corresponding to rPstS-1. c Immunodetection of rPstS-1 from bioreactor supernatants with the polyclonal antibody anti-PstS1, black arrows indicate the immunodetected recombinant protein. The post-induction time for each sample taken is indicated at the top of each lane. M, molecular weight; C−, negative control; supernatant sample from a wild type P. pastoris X-33 culture