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. 2019 Jan 19;18:11. doi: 10.1186/s12934-019-1059-3

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Box plot analysis of antibody reactivity to recombinant antigens rPstS-1. Recombinant proteins (from E. coli and P. pastoris) were tested with PPD-negative (n = 5), PPD-positive sera (n = 5) at 1:100 and active-TB sera (n = 30) at 1:500 dilution. The reactivity is reported as difference of the absorbance units observed for the different group of sera by ELISA using a IgG2 conjugated with AP. Different sera of each group were tested by duplicate. Mean (dashed lane), median, 10th, 25th, 75th and 90th percentiles as vertical boxes with error bars are shown. Dunn´s test ws used for all pairwise comparisons and comparisons against a control group (PBS) following rank-based ANOVA