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Secondary structure of rPstS-1 from P. pastoris and E. coli. Comparative secondary structure content obtained by CD data analysis, NMR data and CAPITO server
| NMR [94] | CAPITO (CD) | ||||
|---|---|---|---|---|---|
| rPstS-1 from E. coli | rPstS-1 from P. pastoris | ||||
| PDB: 1PC3 | 125 μg/mL | 250 μg/mL | 125 μg/mL | 250 μg/mL | |
| Helix (%) | 34 | 16 | 20 | 10 | 5 |
| β-strand (%) | 18 | 28 | 29 | 38 | 40 |
| Irregular (%) | 41 | 50 | 52 | 56 | 53 |
CAPITO CD Analysis & Plotting Tool [93], CD circular dichroism, NMR, nuclear magnetic resonance; PDB protein data bank
