Table 4.
Theoretical and obtained peptides mass generated by Trypsin digestion of rPstS-1 produced in P. pastoris
| Peptide name | Position of cleavage site | Peptide | Length (aa) | Theoretical Peptide mass (Da) | Modification | Peptide mass (Da) |
|---|---|---|---|---|---|---|
| P1T | 54 | AGCGSKPPSGSPETGAGAGTVATTPASSPVTLA ETGSTLLYPLFNLWGPAFHER |
54 | 5315.900 | ND | |
| P2T | 97 | YPQVTITAQGTGSGAGIAQAAAGTVNIGASDAYL SEGDMAAHK |
43 | 4122.489 | B) 4120.98 | |
| P3T | 123 | GLMNIALAISAQQVNYNLPGVSEHLK | 26 | 2781.222 +2 Hex 3105.22 +4 Hex 3429.22 +5 Hex 3591.22 |
P3T + 2 Hex P3T + 4 Hex P3T + 5 Hex |
B) 3105.78 C) 3429.46 C) 3590.15 |
| P4T | 127 | LNGK | 4 | 430.504 | ND | |
| P5T | 138 | VLAAMYQGTIK | 11 | 1194.455 | P5T + 6 Hex | B) 2166.65 |
| P6T | 164 | TWDDPQIAALNPGVNLPGTAVVPLHR | 26 | 2752.125 | B) 2751.45 | |
| P7T | 180 | SDGSGDTFLFTQYLSK | 16 | 1765.894 | C) 1765.82 | |
| P8T | 188 | QDPEGWGK | 8 | 915.958 | ND | |
| P9T | 238 | SPGFGTTVDFPAVPGALGENGNGGMVTGCAE TPGCVAYIGISFLDQASQR |
50 | 4918.459 | C) 4916.29 | |
| P10T | 270 | GLGEAQLGQSSGNFLLPDAQSIQAAAAGFASK | 32 | 3105.411 | B) 3104.56 C) 3105.89 |
|
| P11T | 301 | TPANQAISMIDGPAPDGYPIINYEYAIVNNR | 31 | 3378.761 | B) 3377.64 C) 3379.80 |
|
| P12T | 303 | QK | 2 | 274.320 | ND | |
| P13T | 324 | DAATAQTLQAFLHWAITDGNK | 21 | 2272.502 | B) 2272.13 C) 2271.81 |
|
| P14T | 342 | ASFLDQVHFQPLPPAVVK | 18 | 1993.336 | B) 1993.08 C) 1994.49 |
|
| P15T | 353 | LSDALIATISS | 11 | 1090.239 | P15T + 4 Hex | B) 1738.59 |
Mark of B) and C) referred the mass spectra from Fig. 4b and c, respectively
Hex hexose, ND no determined, T trypsin, aa amino acids