Table 5.
Theoretical and obtained peptides mass generated by Lys-C digestion of rPstS-1 produced in P. pastoris
| Peptide name | Position of cleavage site | Peptide | Length (aa) | Theoretical Peptide mass (Da) | Modification | Peptide mass (Da) |
|---|---|---|---|---|---|---|
| P1 | 6 | AGCGSK | 6 | 521.589 | ND | |
| P2 | 97 | PPSGSPETGAGAGTVATTPASSPVTLAETGSTL LYPLFNLWGPAFHERYPQVTITAQGTGSGAGIA QAAAGTVNIGASDAYLSEGDMAAHK |
91 | 8916.801 | ND | |
| P3 | 123 | GLMNIALAISAQQVNYNLPGVSEHLK | 26 | 2781.222 | ND | |
| P4 | 127 | LNGK | 4 | 430.504 | ND | |
| P5 | 138 | VLAAMYQGTIK | 11 | 1194.455 | ND | |
| P6 | 180 | TWDDPQIAALNPGVNLPGTAVVPLHRSDGSGD TFLFTQYLSK |
42 | 4500.004 | ND | |
| P7 | 188 | QDPEGWGK | 8 | 915.958 | D) 916.41 | |
| P8 | 270 | SPGFGTTVDFPAVPGALGENGNGGMVTGCAE TPGCVAYIGISFLDQASQRGLGEAQLGQSSGN FLLPDAQSIQAAAAGFASK |
82 | 8005.854 | D) 8001.84 | |
| P9 | 303 | TPANQAISMIDGPAPDGYPIINYEYAIVNNRQK | 33 | 3635.065 | D) 3633.80 | |
| P10 | 324 | DAATAQTLQAFLHWAITDGNK | 21 | 2272.502 | D) 2272.13 | |
| P11 | 342 | ASFLDQVHFQPLPPAVVK | 18 | 1993.336 | ND | |
| P12 | 353 | LSDALIATISS | 11 | 1090.239 | ND |
Mark of D) referred the mass spectrum from Fig. 4d
Hex hexose, ND no determined