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. 2019 Jan 18;38:23. doi: 10.1186/s13046-019-1034-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — ITGB4 expression was increased in GSCs. a-c GSC-enriched populations were obtained from LN229 cells by sphere formation assay. The altered genes between spheroid and monolayer were detected by RNA sequencing analysis. The upregulated genes are listed. d-g The expression levels of ITGB4, Oct4, and Nanog were analysed by western blotting and q-RT-PCR assays. Data represent the mean ± SD of three independent experiments. *** p < 0.001 vs. control. h-k ALDH1 positive cells were obtained from LN229 and U251 cells by flow cytometry sorting. The expression levels of ITGB4, Oct4, and Nanog were analysed by western blotting and q-RT-PCR assays. Data represent the mean ± SD of three independent experiments. *** p < 0.001 vs. control