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. 2019 Jan 18;38:22. doi: 10.1186/s13046-019-1039-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — SOX9 rescues the effect of miR-190 on tamoxifen sensitivity and stemness. a, miR-190 expression in indicated MDA-MB-231 cells as determined by RT-qPCR. b, SOX9 expression in indicated MDA-MB-231 cells as determined by western blotting. c~e, Cell growth inhibition was determined by MTT (c), colony formation (d), and EdU (e) assays in MDA-MB-231 cells transfected with miR-190 mimics and SOX9 plasmid, as well as in control cells after treatment with tamoxifen. F, Mammosphere formation assay of cells as in (a). g, CD44^high/CD24^low CSC population analysis of cells as in (a). h, The expression of CSC markers in cells as in (a) was determined by western blotting. *P < 0.05