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. 2019 Jan 18;38:22. doi: 10.1186/s13046-019-1039-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — miR-190 inhibits Wnt/β-catenin signaling by regulating of SOX9. a, Luciferase reporter analysis of Wnt/β-catenin signaling in MDA-MB-231 cells transfected with miR-190 mimics and SOX9 plasmid, as well as in control cells. b, The expression of β-catenin in cells as in (a) was determined by western blotting. C, Localization of β-catenin in cells as in (a) was determined by immunofluorescence staining. d and e, The mRNA and protein expression of downstream targets of Wnt/β-catenin signaling in cells as in (a) was determined by RT-qPCR (d) and western blotting (e). *P < 0.05