KEY RESOURCES TABLE.
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-BrdU monoclonal antibody | Roche | Cat#11296736001 |
Rabbit anti-Ki-67 | Vector Laboratories | Cat#VP-RM04; RRID: AB_2336545 |
Goat anti-AQP2 | Santa Cruz Biotechnology | Cat#sc-9882; RRID: AB_2289903 |
Rabbit anti-phoshoThr53-NCC | PhosphoSolutions | Cat#p1311-53; RRID:AB_2650477 |
Guinea pig anti-NKCC2 | Saritas et al., 2013 | N/A |
Rabbit anti-pT96/T101-NKCC2 | Saritas et al., 2013 | N/A |
Guinea pig anti-pendrin | Wall and Lazo-Fernandez, 2015 | N/A |
Guinea pig anti-parvalbumin | Swant | Cat#GP72; RRID: AB_2665495 RRID:AB_2665495 |
Mouse anti-calbindin D-28k | Swant | Cat#300; RRID: AB_10000347 |
Donkey anti–goat Cy3 | Life Technologies | Cat#A21432; RRID:AB_141788 |
Donkey anti–mouse Cy5 | Life Technologies | Cat#A31571; RRID: AB_162542 |
Donkey anti–rabbit Cy5 | Life Technologies | Cat#A31573; RRID: AB_2536183 |
Chemicals | ||
Fluorescein labeled Lotus Tetragonolobus Lectin | Vectorlabs | Cat#FL-1321; RRID: AB_2336559 |
Propidium iodide | Life Technologies | Cat#P3566 |
Doxycycline | Alfa Aesar | Cat#J60579 |
Citrate-based antigen retrieval solution | Vector Laboratories | Cat#H-3300; RRID:AB_2336226 |
DAPI mounting medium | Thermo Fischer Scientific | Cat#P36971 |
Envision Flex Wash Buffer | Agilent | Cat#K800721-2 |
Antibody diluent | Dako | Cat#S0809 |
Ethanol | Merck | Cat#100983 |
Ethyl cinnamate | Sigma-Aldrich | Cat#112372 |
Acrylamide | Bio-Rad | Cat#161-0140 |
Azo initiator | Wako | Cat#VA-044 |
Paraformaldehyde | Thermo Fischer Scientific | Cat#O4042-500 |
Easy Index clearing solution | Lifecanvas Technologies | Cat#EI-Z1001 |
Experimental Models: Organisms/Strains | ||
Mouse: Pax8-rtTA/LC-Kcnj10fl/fl | Cuevas et al., 2017 | N/A |
Mouse: C57BL/6J | The Jackson Laboratory | Stock No: 000664 |
Software and Algorithms | ||
Imaris | http://www.bitplane.com/imaris/imaris | N/A |
Arivis 4D | https://www.arivis.com/en/imaging-science/arivis-vision4d | N/A |
GraphPad Prism | https://www.graphpad.com/scientific-software/prism/ | N/A |
Other | ||
Potassium-deficient diet | Envigo | Cat#TD.88239 |
Normal potassium diet | Labdiet | Cat#5LOD |