A. Ex vivo analysis of luminescence from lung metastases of LS174T-SGK1 and parental cells, demonstrates only very small lesions in the former.
B. Lung metastasis burden after orthotopic delivery of LS174T-SGK1 is much lower than in parental cells.
C. Immunohistochemical detection of SGK1 in a human CRC tissue microarray shows significantly less expression in poorly differentiated CRCs (p<0.01).
D. Data from TCGA demonstrate that patients with the lowest expression of SGK1 have significantly poorer overall survival (p<0.001).