Figure 4. Atp1b1 targeting contributes to the protective effect of miR-192-5p against hypertension.

A. The level of ATP1B1 in the renal cortex was higher in SS than that in SS.13^BN26 (L26) rats. Western blot signals of ATP1B1 were normalized by Coomassie blue staining of the membrane shown below the Western blot. n=3, *, p< 0.05. B. Na⁺/K⁺-ATPase activity in the renal cortex was increased in SS.13^BN26 rats with intrarenal knockdown of miR-192-5p and in Mir192 KO mice at the end of the blood pressure experiment shown in Figure 3. n=5 Scrambled anti-miR and 6 anti-miR-192-5p; n=5 WT and 6 KO; *, p< 0.05. C. Anti-Atp1b1 GapmeR reduced the level of ATP1B1 based on Western blot analysis of the renal cortex. n=3*, p< 0.05. D. Anti-Atp1b1 GapmeR treatment reduced the activity of Na⁺/K⁺-ATPase. n=3, *, p< 0.05. E. Knockdown of ATP1B1 attenuated hypertension in L26 rats treated simultaneously with anti-miR-192-5p. Anti-miR-192 and anti-Atp1b1 GapmeR or scrambled GapmeR were administered by intrarenal artery injection at the time indicated. n=4, *, p< 0.05 between rats treated with anti-miR-192-5p and anti-Atp1b1 GapmeR and those treated with anti-miR-192-5p and scrambled GapmeR.