. Author manuscript; available in PMC: 2019 Nov 20.

Published in final edited form as: Anal Chem. 2018 Oct 30;90(22):13331–13340. doi: 10.1021/acs.analchem.8b02845

Table 1.

A comparison of mRNA and protein expression level of a selected set of transporters.

Transporter	Protein expression level in monolayer	Protein expression level in crypts	mRNA expression level in monolayer	mRNA expression level in crypts
P-gp/Abcb1a	+++	+++++	+++	+++
BCRP/Abcg2	+	+	++	++
MRP1/Abcc1	+	+	+++	++
MRP3/Abcc3	+	++	+++	+++
NaK ATPase/Atp1a1	++++	++++++	++++	++++

mRNA data was measured at day 5 of culture while protein was measured at day 6 of culture. Protein comparison levels in pmol/mg membrane fraction protein (PL) are PL>50 (++++++); 17.5>PL>15 (+++++); 15>PL>12.5 (++++); 5>PL>2.5 (++); 2.5>PL>0.1 (+). mRNA expression comparison levels in counts per million (mRL) are 3<mRL<4 (++++); 2<mRL<3 (+++); 1<mRL<2 (++); 0.5<mRL<1 (+).