Figure 4.

Normalized Large Neuron Density by Prenatal Treatment.

Serial cross-sections at the MMC defect epicenter for each lamb were imaged and large neurons (LN), defined as cells within the gray matter with a diameter of 30–70 μm, were manually counted. LN density per mm² of gray matter was calculated for each lamb and normalized to average LN density for the corresponding lumbar level of normal lambs. Lambs that were repaired with the High density PMSC-ECM had significantly higher LN density (1.02 ± 0.29% of normal) than those repaired with the ECM vehicle alone (0.45 ± 0.42% of normal).