Figure 1: Overview of the rapid 3D bioprinting process to fabricate dECM tissue constructs with tissue-specific hiPSCs.

Human iPSCs were differentiated into hiPSC-CMs and hiPSC-Heps then subsequently combined with their respective tissue-specific photocrosslinkable dECM bioinks. Digital patterns recapitulating key histological features of each tissue were designed and inputted into a custom-built rapid DLP-based 3D bioprinter to create tissue constructs possessing microscale biomimetic microarchitecture in the order of seconds. This approach can be readily extended to create a wide range of tissue-specific photocrosslinkable dECM bioinks to build personalized human tissue platforms for broad applications in tissue engineering and regenerative medicine.