Figure 4. Bioprinting of biomimetically patterned heart and liver dECM tissue constructs.

(a) Designed biomimetic digital patterns and (b) their respective acellular 3D bioprinted heart and liver tissue constructs. Scale bar, 1 mm. Microstructural features of the printed (c) acellular and (d) cellularized heart and liver tissue constructs. Scale bar, 200 μm. (e,f) Macroscopic images of the bioprinted dECM tissue constructs. (g,h) To evaluate the long term stability of acellular bioprinted dECM tissue constructs and their respective collagen I controls, samples were immersed in Ringer’s physiological solution at 37°C and the swelling ratio was measured over 28 days. All data are expressed as mean ± standard deviation. (n=3).