Figure 6: Phenotype and gene expression profile of tissue-specific markers of hiPSCs cultured in the bioprinted dECM tissue constructs after 7 days.

Representative confocal images of immunohistochemical staining of the bioprinted (a) heart (α-actinin, actin) and (b) liver (E-cadherin (E-cad), albumin (ALB)) tissue constructs and their respective collagen I controls. All samples were counterstained with DAPI nucleic dye. Scale bar, 50 μm. (n=4). Gene expression of early and late tissue-specific markers for the bioprinted (c) heart and (d) liver tissue constructs relative to collagen I controls. All values were normalized to 2D controls and GAPDH served as the housekeeping gene. All data are expressed as mean ± standard deviation. * = significant to collagen I and 2D controls; ** = significant to 2D controls, (p< 0.05). (n=3).