Figure 4.

Gating strategy for γδ T cells. Single-cell suspensions were first gated for physical parameters, including forward scatter (FSC), a measure of size, and side scatter (SSC), a measure of cell granularity. γδ T cells were selected as positive for expression of CD3, a coreceptor expressed by all T cells, followed by gating on those also expressing TCRγ/δ, represented as shaded areas. Activated γδ T cells were identified based on their increased expression of CD69. Appropriate isotype controls are shown in gray with stained samples in blue.