Figure 5.

Flow cytometry reveals a significant variation in γδ T cell numbers and activation states across three skin sites. There are significantly fewer γδ T cells, which also show increased CD69 expression, in the footpad relative to the ear and back. (a) The percentage of CD3+ cells expressing TCRγ/δ is significantly greater in the ear and back than in the footpad. (b) The activation state of CD3 + TCRγ/δ + cells, assessed using the cell-surface antigen CD69, shows increased expression among cells in the footpad than in the ear and back. Bar graphs represent mean ± SEM (^∗∗ p < 0.001 and ^∗ p < 0.01, one-way ANOVA with post-hoc Tukey test; n = 4/group).