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. 2019 Jan 13;11(1):185–208. doi: 10.18632/aging.101740

Figure 7.

Figure 7

Rapamycin causes a reversible effect on spermatogenesis. All panels show samples from adult males injected with vehicle control (ctrl-rec, n=3) or rapamycin for 3 weeks (rapa-rec, n=3), followed by a recovery period of 2-months. (a) Gross testis morphology. (b-d) Testis mass (b), sperm count (c), and sperm motility (d). Data represent the mean ±SD (NS, not significant, **P<0.01, Student’s t test). (e-h) Testis histology reveals full spectrum of spermatogenesis at 2 months after rapamycin treatment cessation. (i) Western blot analysis of phosphorylation of mTORC1 and mTORC2 substrates.