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. Author manuscript; available in PMC: 2019 Oct 19.
Published in final edited form as: J Mol Biol. 2018 Sep 15;430(21):4401–4418. doi: 10.1016/j.jmb.2018.09.007

Figure 9.

Figure 9.

LI attP substitutions resulting in high integration efficiencies. In vitro integration time courses were carried out as in Fig. 6. A) Examples of reaction time courses for the 2T, 3C, and 7C mutants. B) Integration kinetics of hyperactive mutants vs. wild-type attP. Error bars are standard deviations based on three independent experiments. The inset graph shows early time points. Rapid product formation is evident from the zero time point, where reactions were quenched within 1–3 sec after initiation.