Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 14;6:211. doi: 10.3389/fbioe.2018.00211

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Schmieg, Döbber, Kirschhöfer, Pohl and Franzreb.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Overview of the studied reactions. (A) Hydrolysis of o-Nitrophenyl-β-d-galactopyranoside (ONPG) by β-Gal yields the monosaccharides galactose and o-nitrophenol. (B) Activity of ADH can be determined by enantioselective reduction of acetophenone to (R)-phenylethanol. (C) The combination of BFD and ADH in a 2-step cascade reaction. Carboligation of the educts benzaldehyde and acetaldehyde catalyzed by BFD yields the intermediate (S)-2-hydroxy-1-phenyl-propanone ((S)-HPP), which can be further reduced to the product (1S,2S)-1-phenylpropane-1,2-diol ((S,S)-PPD) by ADH. The redox equivalents are delivered by the cofactor NADPH that is oxidized to NADP⁺. For in situ regeneration of NADPH 2-propanol was added in excess which is oxidized to acetone by the same ADH.