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. 2019 Jan 14;6:211. doi: 10.3389/fbioe.2018.00211

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Copyright © 2019 Schmieg, Döbber, Kirschhöfer, Pohl and Franzreb.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detected conversion to the respective product compound at the exit of the 3 ml reactor system of one exemplary run. Parameters were (A) β-Gal (m = 0.38 mg/reactor) with ONPG substrate 2.2 mM, flow rate 0.05 ml/min. (B) BFD (m = 56 mg/reactor) catalyzing the carboligation of benzaldehyde (25 mM) and acetaldehyde (62.5 mM), flow rate 0.017 ml/min. (C) ADH (m = 42 mg/reactor) reducing acetophenone (50 mM), flow rate 0.017 ml/min. Reproducibility is shown by a second experiment with identical parameters. (D) ADH (m = 58 mg/reactor) reducing (S)-HPP provided by BFD reactor experiments (11 mM), flow rate 0.017 ml/min.