Table 2.

Overview of the used parameters for enzyme kinetics and the calculated kinetic parameters for experiments in solution and hydrogel lattices.

Applied enzyme		β-Gal	BFD	ADH aceto phenone	ADH cascade
FREELY DISSOLVED ENZYMES
cenzyme/crudecellextract, solution	g l−1	0.01	1	1	1
csubstrate	mmol l−1	2.2	35	39	4.8
vreaction, solution	mmol l−1min−1	0.08	1.15	2.09	0.016
kapp, solution, experimental	min−1	0.036	0.033	0.053	0.0034
vmax (with 95% confidence bounds)	mmol l−1min−1	0.13 (0.12, 0.14)	2.81	3.03 (2.77, 3.28)	0.02 (0.02, 0.03)
Km (with 95% confidence bounds)	mmol l−1	1.40 (1.12, 1.68)	Km, acetaldehyde 44.04	17.55 (14.09, 21.01)	2.35 (1.75, 2.95)
			Km, benzaldehyde 21.66
ENTRAPPED ENZYMES
cenzyme, hydrogel referred to Vsolution	g l−1	0.011	6	4.4	4.4
cenzyme, hydrogel referred to Vhydrogel	g l−1	0.25	40.7	26.2	34.9
csubstrate	mmol l−1	2.2	35	44	4.8
Vsolution	ml	6	4.1	1.7	1.6
Vhydrogel	ml	0.26	0.6	0.28	0.2
vreaction, hydrogel referred to Vsolution	mmol l−1min−1	0.006	0.41	0.91	0.01
vreaction, hydrogel referred to Vhydrogel	mmol l−1min−1	0.137	2.821	5.470	0.080
kapp, hydrogel, experimental	min−1	0.062	0.081	0.124	0.017
kapp, hydrogel, theoretical	min−1	0.894	1.343	1.403	0.120
Effectiveness factor η	%	6.9	6.0	8.9	14.0