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. 2019 Jan 14;12:519. doi: 10.3389/fncel.2018.00519

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Copyright © 2019 Monaco, Baur, Hellwig, Hölzl-Wenig, Mandl and Ciccolini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A flow cytometric approach for the isolation and characterization of primary and motile cilia. Flowchart of the experimental procedure for cilia identification and characterization according to size and marker expression. The * symbol represents a conjugated fluorophore. (A,B) FACS plots showing the distribution of reference beads and a sample of cilia according to forward (FSC) and side (SSC) scatter. (C) Representative FACS plot illustrating the distribution of 2 μm particles upon immunostaining with antibodies directed against adenylate cyclase type iii (AC3; AC3-FITC) and prominin-1 (Prom-APC). The gates indicate AC3 positive (AC3+), Prominin positive (Prom+) and double positive (DP) particles.