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. 2019 Jan 14;12:519. doi: 10.3389/fncel.2018.00519

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Copyright © 2019 Monaco, Baur, Hellwig, Hölzl-Wenig, Mandl and Ciccolini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cilia characterization according to the expression of AC3 and prominin-1 in different mouse ages. (A) Representative FACS plots illustrating the distribution of particles of different sizes according to AC3 and prominin-1 (Prom) staining in E18 embryos, 8 week-old (8w) and 25 week-old (25w) mice. (B–D) Quantification of AC3⁺, DP and Prom⁺ particles in at least three independent experiments. Values represent the average percentage of particles in each population. Error bars represent SEM. Statistically significant differences are indicated with asterisks (**p < 0.01, ***p < 0.001, ****p < 0.0005). (E) Schematic representing the relative expression of AC3 and Prom across ages.