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. 2019 Jan 14;12:519. doi: 10.3389/fncel.2018.00519

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Copyright © 2019 Monaco, Baur, Hellwig, Hölzl-Wenig, Mandl and Ciccolini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Platelet-derived growth factor receptor α (PDGFRα) and CXC chemokine receptor 4 (CXCR4) expression in primary cilia. (A,C) FACS histograms depicting PDGFRα and CXCR4 total expression in particles of different size in E18 embryos (E18), 8 week-old (8w)- and 25 week-old (25w) mice. The gates include PDGFRα-PE or CXCR4-PE positive particles. (B,D) Quantification of the average percentage of PDGFRα⁺ and CXCR4⁺ particles in at least three independent experiments. (E,F) Quantitative analysis of the co-localization of PDGFRα and CXCR4 with AC3 (green bars), prominin-1 (Prom; red bars) and both AC3 and Prom (blue bars), as percentage of PDGFRα⁺ or CXCR4⁺ total particles. Values represent the average percentage of particles in each population. Error bars represent SEM. Statistically significant differences are indicated with asterisk (*p < 0.05, **p < 0.01, ***p < 0.001).