FIGURE 1.

Chlamydia trachomatis EB bind to the surface of Candida albicans. FBS-coated coverslips were inoculated with C. albicans or medium alone and incubated 3 h prior to exposure to EB (Ca/CtE or CtE) or 2SPG (Ca). Following incubation of 1 h, the cultures were washed, replenished with medium and incubated for 24 h before collection for IFA by fixation with methanol or formaldehyde (No permeabilization). Samples were stained with Pathfinder anti-Chlamydia MOMP stain and visualized 100× magnification.