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. 2019 Jan 14;9:3270. doi: 10.3389/fmicb.2018.03270

FIGURE 2.

FIGURE 2

Confocal and transmission electron microscopy imaging of C. albicans/C. trachomatis cultures. C. albicans hyphal cultures were prepared alone or exposed to EB and processed for IFA and TEM visualization as described in the methods. (A) Confocal image of Ca/CtE culture at 100x magnification. (B) Individual Z sections taken at 30 micron intervals throughout the depth of the sample. Bottom denotes the coverslip. (C) Cross-section of the confocal image presented in (A). Green: Pathfinder anti-Chlamydia MOMP stain, Red: Aniline blue stain for Candida. (D) TEM micrograph of Ca culture at 7000× magnification. (E) TEM micrograph of Ca/CtE culture at 7000× magnification. (F,G) TEM micrographs of Ca/CtE cultures at 20000× magnification. Yellow Arrows: C. trachomatis EB, White Arrows: Candida cell wall.