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. 2019 Jan 14;9:3270. doi: 10.3389/fmicb.2018.03270

FIGURE 3.

FIGURE 3

Binding to Candida albicans decreases C. trachomatis EB infectivity. FBS-coated coverslips were inoculated with C. albicans or medium alone and incubated 3h prior to exposure to EB (Ca/CtE or CtE) or 2SPG (Ca). Following incubation of 1h, the cultures were washed, replenished with MEM or M199 medium ± G6P/FBS. Duplicate sets of samples were fixed and processed for IFA or harvested for EB titer analysis at various times post incubation. (A) IFA of Ca/CtE at Day 0, 1, and 3 post incubation in M199 ± G6P/FBS. Chlamydia EB -Red: mouse anti-Chlamydia MOMP/rabbit-anti-mouse Alexa Fluor 594, Candida: DIC 200x magnification. (B) EB titers from Ca, CtE and Ca/CtE cultures at Day 0, 1, and 3 post incubation in M199 ± G6P/FBS. EB titers form CtE and Ca/CtE cultures harvested at 0 and 6h post incubation in MEM (C) or MEM+G6P/FBS (D). (E) Percentage of IFU/ml remaining 6h post incubation compared to 0h for CtE and Ca/CtE cultures in MEM ± G6P/FBS. The data shown represent the means ± SEM of three independent repeats with 3 biological replicates/repeat (n = 9). An asterisk (∗) indicates a significant difference between CtE and Ca/CtE (p ≤ 0.05) as determined by ANOVA and two-sample independent T-tests.