Figure 2.

(A) Schematic map of pOGG024 level 1 broad-host range, medium copy number vector constructed with BEVA for free-living gene expression in laboratory. (B) Schematic map of plasmid pOPS0359 containing a cloned gene sfGFP under the control of a taurine-inducible promoter from Sinorhizobium meliloti in pOGG024. (C–F) Evaluation of pOPS0359 performance as robust free-living gene expression compared to the functionally analogous pLMB509 plasmid. (C,D) Fluorescence detection from GFP expression and (E,F) fitness performance by measurement of OD₅₉₅ when grown without (0 mM) or with (0.5, 1.0, 10, or 40 mM) taurine. For all graphs, error bars are standard error of the mean (SEM).