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. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345

Figure 5.

Figure 5

(A) Schematic map of pOGG216 level 2 broad-host range, medium copy number vector constructed with BEVA for multi-gene expression. (B) Schematic map of dual reporter plasmid pOPS0754 which encodes IPTG-inducible sfGFP cloned in Forward Position 1 and constitutively mCherry cloned in Forward Position 2. (C,E,G) Green fluorescence detection (scale, 5,000–31,000). (D,F) Red fluorescence detection (scale, 3,000–29,000 cps). (C,D) Rlv3841 without fluorescence. Rlv3841[pOPS0754] expressing IPTG-inducible sfGFP grown in media (E) containing 0.5 mM IPTG or (G) without IPTG. (F) Rlv3841[pOPS0754] with constitutively expressed mCherry.