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. 2019 Jan 14;9:2003. doi: 10.3389/fpls.2018.02003

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Copyright © 2019 Feng, Wang, Wu, Luo, Zhang, Qiu, Han, Su, Xiong, Chang, Yang and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein interaction analyses between TaSnRK2.9 and NtABF2 by yeast two-hybrid assay and expression analyses of the related genes. (A) TaSnRK2.9 was cloned into the vector pGADT7, and three NtABFs were transferred to vector pGBKT7, then they were co-transformed into the yeast strain AH109. The transformants were grown on nutritional selective medium SD/-Trp-Leu, or SD/-Trp-Leu-His-Ade, or plus the chromogenic substrate X-α-Gal. The first and second lines indicate the positive and negative control, respectively. Three independent biological experiments were performed and produced similar results. (B,D) The relative expression level of the predicated interacting gene NtABF2 under drought or salt stress in tobacco. (C,E) The relative expression level of the related gene NtMYB102. Data in chart are means ± SD (n = 3, ^∗P < 0.05; ^∗∗P < 0.01 by Student’s t-test).