Figure 5.

Crosslinking of NKG2A dampens Ca²⁺ flux triggered by NKp46 stimulation in human 6 human donors. (A) Kinetics plot of Fluo-8 fluorescence of CD57 low, CD56⁺, NKG2A⁺ NK cells stained with biotinylated anti-NKp46 and biotinylated anti-CD94 and co-crosslinked using by Streptavidin. HD1-HD6 depict the 6 donors. Different concentrations of anti-NKG2A are color-coded. (B,D) Total AUC values of CD56⁺ NKG2A⁺ CD57 low NK cells, after baseline correction, of the data in (A). Statistics were calculated using a paired Student's T-test for the combinations indicated. (C) The fractions of CD57high/CD57low NK cells from HD1-HD6. Gating as indicaed in Figure S2. (D) Similar data as in (B) but for CD56⁺ NKG2A⁺ CD57 high NK cells. (E) Total AUC values of CD57 high and CD57 low NK cells stimulated with biotinylated anti-NKp46 and crosslinked with Streptavidin, in the absence of inhibitory receptor crosslinking. Statistics were calculated using a parid Student's T-test. (F) The inhibition index obtained by streptavidin-mediated crosslinking of NKG2A⁺ NK cells at lowering concentration of biotinylated anti-CD94, and comparing CD57 low and CD57 high NK cells. No significant differences were seen. ^*p < 0.05, ^**p < 0.01, ^****p < 0.0001, ns, not significant.