FIGURE 1.
Changes to body weight, temperature, blood glucose levels and inflammatory responses following LPS administration in WT and sEH null mice. (A) Differences in body weight of WT and sEH null mice with or without injection of LPS, (B) differences in body temperature in WT and sEH null mice with or without injection of LPS, (C) comparing blood glucose levels for WT and sEH mice with or without injection of LPS, (D) serum levels of TNFα in WT and sEH null mice, (E) serum levels of MCP-1 in WT and sEH null mice, (F) DNA binding activity of NF-kB in hearts from WT and sEH null mice. Representative immunoblots and densitometric quantification of (G) sEH (60 kDa), (H) eNOS, (135 kDa), and (I) TLR4 (95 kDa) protein expression in hearts from WT and sEH null mice. All expression was normalized to GAPDH loading control. Data presented as mean ± SEM, N = 4–6. Statistics: Two-way ANOVA with Tukey’s post hoc test. ∗p < 0.05 vs. control, #p < 0.05 vs. LPS treatment groups.