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. 2019 Jan 14;9:3172. doi: 10.3389/fimmu.2018.03172

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Copyright © 2019 Rojas, Rodríguez-Martín, Avia, Martín and Sevilla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PPRV-P, -F, and -H expression in transfected cells. (A) Vero cells were grown on coverslips, transfected with pCAGG-Empty, -PPRV-F-HA, -PPRV-H-HA, or pIRES-PPRV-P-FLAG, and protein expression assessed using anti-tag antibodies (HA/FLAG). Nucleic acids were visualized by DAPI staining. Representative confocal images of transfected cells are shown. Scale bar = 20 μm. (B) 293T cells were mock-transfected (Mock) or transfected with pCAGG-Empty, -PPRV-F-HA, -PPRV-H-HA or pIRES-PPRV-P-FLAG, and cell lysate obtained. Proteins were resolved on 10% SDS-PAGE, transferred to nitrocellulose membrane and probed (IB) for protein expression using anti-tag antibodies (HA/FLAG). Membranes were also probed with anti-GAPDH antibody as loading control. Arrowheads indicate protein bands at the predicted molecular weight for PPRV-H (70 kDa), PPRV-F (59 KDa), and PPRV-P (75–79 kDa depending on phosphorylation).