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. 2019 Jan 14;9:3172. doi: 10.3389/fimmu.2018.03172

Figure 5.

Figure 5

ADCC is directed to PPRV-F and -H proteins. 293T cells were mock-transfected, transfected with an empty expression plasmid or PPRV -P, -F, or -H expressing plasmids. (A) Transfected 293T cells were incubated with anti-PPRV immune serum and anti-sheep IgG-alexa488 secondary antibody. Sheep IgG binding to transfected cells was evaluated by flow cytometry. Representative flow cytometry histograms from three independent experiments are shown. (B) Transfected 293T cells were incubated with PPRV immune serum and used as target cells. ADCC was measured by flow cytometry cytotoxicity assays using IL-2-expanded ovine NK cells (>40% CD16+ cells) from donor sheep as effector cells. Specific lysis (mean ± SD) in ADCC assays performed in four donor sheep at different effector to target cell ratio (E: T) are shown. *p < 0.05; **p < 0.01; ***p < 0.001 Two-way ANOVA with Bonferroni's post-test (PPRV-P, -F, or -H vs. empty).