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. 2019 Jan 14;12:515. doi: 10.3389/fncel.2018.00515

Figure 1.

Figure 1

CDDP induces cell death and increases both sirtuin 1 (SIRT1) and autophagy in HEI-OC1 cells. (A) The CCK8 assay was performed to examine cell viability of HEI-OC1 cells in CDDP (20 μM) exposure from 0 h to 48 h (n = 3 individual experiments). (B) The CCK8 assay was performed to examine cell viability of HEI-OC1 cells in CDDP exposure from 0 μM to 100 μM for 24 h (n = 3 individual experiments). (C,D) Apoptosis measured by annexin V and propidium iodide (PI) staining for HEI-OC1 cells in CDDP (20 μM) exposure for 24 h (n = 3 individual experiments) and its analysis. (E,F) Western blots and densitometry analysis for SIRT1 and autophagy marker LC3-II and p62 in CDDP (20 μM) exposure for 24 h (n = 3 individual experiments). Data represent the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. CDDP, cisplatin.