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. 2019 Jan 14;12:515. doi: 10.3389/fncel.2018.00515

Figure 6.

Figure 6

Autophagy activation attenuates cisplatin-induced hair cell loss and hearing loss in C57BL/6 mice. (A) Auditory brainstem response (ABR) thresholds decreased with RA (7.5 mg/kg, intraperitoneal injection pre-24 h, pre-1 h, post-24 h) treatment mice in CDDP (16 mg/kg, intraperitoneal injection) exposure compared with the CDDP groups at 4, 8, 16 and 32 kHz (n = 6 mice). (B,C) Surface preparations were stained with 4′,6-diamidino-2-phenylindole (DAPI). Hair cell counts obtained for the CDDP and CDDP+RA group (n = the right cochlea of six mice). Scale bar, 10 μm. Data represent the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Control, mice were intraperitoneally injected with the same dose DMSO as the CDDP group; RA, mice were intraperitoneally injected with rapamycin three times; CDDP, mice were intraperitoneally injected with CDDP; CDDP+RA, mice were intraperitoneally injected with CDDP once and rapamycin three times. CDDP, cisplatin; RA, rapamycin; Apical, the apical turn; Middle, the middle turn; Basal, the basal turn; Red asterisks, lost hair cells.