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. 2019 Jan 14;9:1889. doi: 10.3389/fphys.2018.01889

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Copyright © 2019 Palstra, Mendez, Dirks and Schaaf.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental set-ups. Experiment I was executed with wild-type (wt) zebrafish with a control group sampled at the start of the experiment, a non-exercised group and an exercised group which was subjected to forced sustained swimming at the optimal swimming speed (Uopt) of 0.4 m s^-1 for a period of 4 weeks in a swim tunnel. Experiment II had a similar set-up but was executed with wild-type zebrafish and Gr mutants. Now there were three exercise groups swimming at the sub optimal (<Uopt), optimal, and super optimal (>Uopt) swimming speeds of 0.3, 0.4, and 0.5 m s^-1, respectively.