FIG 4.

Transfer of infectious virus to HFF. (A) VZV-infected monocytes were isolated by FACS and seeded onto uninfected HFF monolayers for 5 days. The results of immunofluorescence assay (IFA) staining for early VZV pORF29 and late VZV gE shows infectious centers are presented. A merged IFA image of VZV-infected monocytes inoculated on HFF monolayers is shown. Individual channels show pORF29 (green), gE (red), DAPI (blue), and a merged image of mock-infected monocyte inoculation. All images were taken at a ×20 magnification and are representative of those from 3 independent experiments. (B) VZV-infected monocytes were pretreated with citrate buffer prior to inoculation onto uninfected HFF monolayers. A merged IFA image of citrate buffer-treated VZV-infected monocytes inoculated on HFF monolayers is shown. Individual channels of VZV gE (red) and DAPI (blue) are depicted. All images were taken at a ×20 magnification and are representative of those from 3 independent experiments. (C) Enumeration of infectious centers observed following inoculation of untreated and citrate buffer-treated, VZV-infected monocytes onto uninfected HFF. Each symbol represents an independent donor.