FIG 4.

IFN-α enhances the suppressive capacity of HD and ES NK cells. NK cells from 8 HD (A) and 10 ES (B) were treated with either medium alone (NT) or various concentrations of IFN-α for 6 h, washed, and then cocultured over 3 days at a 1:1 ratio with autologous CD4⁺ T cells infected with a GFP-tagged pseudotyped lab strain of HIV-1. Viral replication suppression was measured as a percentage of GFP expression via flow cytometry. Triplicates were performed for each patient, with error bars representing the standard deviation, from means within treatment groups. One-way repeated-measures ANOVA was used to determine the difference between baseline and specific treatment groups. Asterisks indicate the differences from a no-treatment baseline control (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).