FIG 3.

Detection of EV-A71 in blood and in various organs in humanized mice after infection. Four-week-old humanized mice were inoculated i.p. with or without EV-A71 (10⁸ PFU/mouse). (A) Viral titers in the blood of surviving mice (n = 4) and moribund mice (n = 4) were determined by qPCR. Data are expressed as means ± SEM. n.s., not significant. (B) Viral titers in various organs of surviving mice (n = 4) and moribund mice (n = 4) at different endpoints were determined by qPCR. (C and D) Viral titers in different organs of surviving mice (n = 2 for each time point) and moribund mice (n = 2 for each time point) at 4 weeks (C) and 6 weeks (D) after EV-A71 infection are shown. Data are expressed as means ± SEM. *, P < 0.05; **, P < 0.01.