FIG 7.

Infiltration of human CD45⁺ immune cells in various organs in EV-A71-infected humanized mice. Four-week-old humanized mice were inoculated i.p. with or without EV-A71 (10⁸ PFU/mouse). The total number of different immune cell types in the brain (A) and the lung (B) of the mock-infected (n = 4) and EV-A71-infected humanized mice (n = 4) is indicated in the graphs. Data are expressed as means ± SEM. *, P < 0.05; **, P < 0.01. (C) Representative photomicrographs of immunohistochemistry for different organs among mock-infected mice (n = 5), EV-A71-infected surviving mice (n = 6), and moribund mice (n = 4) are shown. Only a few human CD45⁺ immune cells (black arrow) were present in the lung of the mock-infected mice, while the EV-A71-infected mice showed a marked increase in the number of infiltrating human CD45⁺ cells in brain, spinal cord, and lung. The boxes depict magnified areas. Bars, 100 μm.