TABLE 5.
HHV-6B ORF U38 detection by RNA-seq and RT-qPCR in post-HCT whole-blood samples
| Subject | Sample description | U38 reads, RNA-seqa | U38 RNA copies/ml, RT-qPCR | U38 DNA copies/ml, qPCRb |
|---|---|---|---|---|
| Case 3 | Whole blood | 8 | 19c | 0 |
| Case 4 | Whole blood | 0 | 754 | 0 |
| Case 5 | Whole blood | NDd | 78 | 0 |
| Case 7 | Whole blood | 102 | 106,188 | 213 |
| Control 1 | Whole blood | 0 | 0 | 0 |
| Control 2 | Whole blood | 0 | 0 | 0 |
| Control 3 | Whole blood | 0 | 0 | 0 |
| Control 4 | Whole blood | 0 | 0 | 0 |
| Control 5 | Whole blood | 0 | 0 | 0 |
| B-LCL 1 | Postnatal HHV-6B infection | 0 | 0 | 0 |
| B-LCL 2 | iciHHV-6B | 0 | 30.4e | 30.1e |
a
Absolute read count (from Table S1).
b
DNA contamination was assessed by testing the RNA samples without performing a reverse transcription step to identify background cDNA.
c
This value corresponds to a detection level of approximately 6 copies per reaction, which is within the detection limit of the assay.
d
There was insufficient RNA isolated to allow for RNA-seq.
e
This result is expressed as the cycle threshold, not copies per milliliter. The cycle threshold values of the assays with and without reverse transcriptase (to demonstrate U38 RNA and DNA, respectively) were identical, which indicated that the U38 signal was from residual contaminating DNA.