FIG 2.

RNAi knockdown of candidate causal genes in the drh-1 mutant strain. Shown are results of real-time qRT-PCR of Orsay virus RNA2 levels 2 days after Orsay virus infection of animals fed RNAi against independent genes. Values are means plus standard deviations (error bars) for three independent biological experiments, each with three replicate wells. Statistically significant differences were determined by one-way analysis of variance (ANOVA) [F(3,32) = 14.47; P < 0.0001], with statistical difference identified between empty vector and drl-1 (P = 0.0001 by Dunnett’s multiple-comparison test). ****, P < 0.0005; NS, not significant (P > 0.05).