FIG 7.
Overexpression of gp83 may reduce dorsal levels. (A) Western blots show endogenous dl protein levels in S2 cells transfected with a plasmid encoding gp83 or empty control plasmid (left) and in S2 cells infected with KV (4 dpi) (right). The Toll pathway was activated by expression of pAc-TollLRR, as indicated. Western blot analysis using anti-tubulin antibody was used to verify equal loading. (B and C) The effect of gp83 was analyzed by confocal microscopy of S2 cells transfected with plasmid encoding gp83 or control plasmid and plasmids encoding either GFP or dl-GFP. ImageJ-based quantification of mean GFP fluorescence for individually outlined cells is shown (n ≥ 20 cells for each condition; error bars show SEMs). (C) A representative image from panel B, showing GFP (top) and dl-GFP (bottom) expression with or without gp83. Nuclei were visualized using Hoechst.