TABLE 1.

Primers for cloning and dsRNA synthesis^a

Use and source	Amplicon or targeted gene	Primer F name	Primer F sequence	Primer R name	Primer R sequence
Cloning
KV	Putative protein 12	PutPro12_Acc651_0F	actgGGTACCaacATGATCAACCACCAAGGTATCG	PutPro12_XbaI_273R	tgacTCTAGAATATTCCAGTTTTATCATCAGATGTTTG
KV	Putative protein 15	PutPro15_Acc651_0F	actgGGTACCaacATGTCTTGCAATAAAGTCGAATC	PutPro15_XbaI_819R	tgacTCTAGAGATTTTAGATGGTTTGCGGTTA
KV	Putative protein	PutPro_Acc651_0F	actgGGTACCaacATGTTCAAGTATTCAAACTCAAAATA	PutPro_XhoI_378R	tgacCTCGAGATTTGATGAATATCCTTGATCTAAAAG
KV	Putative serine protease	PutSerProt_Acc651_0F	actgGGTACCaacATGTTGCCAATTATAAGTTCG	PutSerProt_XbaI_960R	tgacTCTAGAAACATTTGATTGGCAAATG
KV	gp23-like	gp23-like_Acc651_0F	actgGGTACCaacATGGCGGACAAAAAAATATTC	gp23-like_XbaI_1878R	tgacTCTAGATTCCATTTTTAAACGTTTACTCTG
KV	gp83-like	gp83-like_Acc651_0F	actgGGTACCaacATGTCAGAATCAAAGCTGCAAC	gp83-like_XbaI_1278R	tgacTCTAGATTTCTTATTATCGGATTTTTTCAATG
KV	ach96233-like	ach96233_Acc651_0F	actgGGTACCaacATGCGTGTGTATAGACCTACAATGAAG	ach96233_XbaI_3009R	tgacTCTAGAGGGAAAAATGCTTACAACTGTCG
KV	ach96143-like	ach96143_Acc651_0F	actgGGTACCaacATGACTTCGACAACCGTTAAC	ach96143_XbaI_585R	tgacTCTAGAGATTTTATCGTATATCTTCTTAATTACTTTTTC
KV	gp72-like	gp72-like_BsiWI_0F	actgCGTACGaacATGAACGTTTCAATTGGAAATATTAGC	gp72-like-XhoI_662R	tgacCTCGAGAGCTACGGCCACTGGTCTTG
KV	gp43-like	gp43-like_BsiWI_0F	actgCGTACGaacATGAGTACATTGTTGAATTTGTGCG	gp43-like_XbaI_1296R	tgacTCTAGACAAAAGTATATTATCAAATACATCATCATTGTTG
KV	gp83-delta1 fragment 1	gp83-like_Acc651_0F	actgGGTACCaacATGTCAGAATCAAAGCTGCAAC	gp83_del1_R	ACTAACCGATATTGCAATTAATTGCTTTCG
KV	gp83-delta1 fragment 2	gp83_del1_F	CGAAAGCAATTAATTGCAATATCGGTTAGT	gp83-like_XbaI_1278R	tgacTCTAGATTTCTTATTATCGGATTTTTTCAATG
KV	gp83-delta2 fragment 1	gp83-like_Acc651_0F	actgGGTACCaacATGTCAGAATCAAAGCTGCAAC	gp83_del2_R	TTTTGTACGGAATCGAGACATGCGATCGTA
KV	gp83-delta2 fragment 2	gp83_del2_F	TACGATCGCATGTCTCGATTCCGTACAAAA	gp83-like_XbaI_1278R	tgacTCTAGATTTCTTATTATCGGATTTTTTCAATG
DiNV	DiNV gp83	DiNV_gp83_Acc651_0F	actgGGTACCaacATGGATAGCAAAACAGAAACAAC	DiNV_gp83_XbaI_1350R	tgacTCTAGACAATCCTGCTTTATTATCGG
Dmel	cact	cact_Acc651_0F	actgGGTACCaacATGCCGAGCCCAACAAAAG	cact_XhoI_1503R	tgacCTCGAGGGCAACTGTCATGGGATTG
Dmel	Dif	Dif_Acc651_0F	actgGGTACCaacATGTTTGAGGAGGCTTTCG	Dif_XbaI_2001R	tgacTCTAGATTTGAATGGCTGAATTCCC
Dmel	dorsal	dl_Acc651_0F	actgGGTACCaacATGTTTCCGAACCAGAAC	dl_XbaI_2031R	tgacTCTAGACGTGGATATGGACAGGTTC
Dmel	pelle	pll_Acc651_0F	actgGGTACCaacATGAGTGGCGTCCAGAC	pll_XbaI_1503R	tgacTCTAGAGTCGGTAACAAACGGTTC
Dmel	tube	tub_Acc651_0F	actgGGTACCaacATGGCGTATGGCTGGAAC	tub_XbaI_1386R	tgacTCTAGATTGCTGCAGCTCACTCAAG
dsRNA synthesis
Dmel	msr-110	msr-110_T7_210F	taatacgactcactatagggATCCTTCATCCTGGCCTCCT	msr-110_T7_627R	taatacgactcactatagggCGAGTCGACAATCTCCTCGG
Dmel	msr-110	msr-110_T7_608F	taatacgactcactatagggCGAGTCGACAATCTCCTCGG	msr-110_T7_1127R	taatacgactcactatagggTGGATCTCACGGAAGGGGAT
Dmel	brf	brf_T7_921F	taatacgactcactatagggAGATATGGGCGAGCATGAGC	brf_T7_1248R	taatacgactcactatagggGGTCACCCTGCAAATAGCCT
Dmel	brf	brf_T7_1229F	taatacgactcactatagggAGGCTATTTGCAGGGTGACC	brf_T7_1734R	taatacgactcactatagggTGCCCTATTGCCCCTACTCT
Dmel	Nipped-B	NippedB_T7_1390F	taatacgactcactatagggGTGAAGGAGGAAGTCACCCG	NippedB_T7_1876R	taatacgactcactatagggCCGTGTAAACCGGGTCATGA
Dmel	Nipped-B	NippedB_T7_5103F	taatacgactcactatagggATTCGGGCTACAGCTTTGCT	NippedB_T7_5555R	taatacgactcactatagggTCCGTCAAATCTTCGGGCAA
Dmel	RhoGEF2	RhoGEF2_T7_557F	taatacgactcactatagggACAATGCGAGCCACAACAAC	RhoGEF2_T7_1036R	taatacgactcactatagggCTGATACCGAAGAGGGTCGC
Dmel	RhoGEF2	RhoGEF2_T7_4521F	taatacgactcactatagggGGAGAGCGAGGATGAAGACG	RhoGEF2_T7_4906R	taatacgactcactatagggCCGCAAACTCGCAAAGAACA
Dmel	singed	singed_T7_88F	taatacgactcactatagggATCAACGGCCAGCACAAGTA	singed_T7_511R	taatacgactcactatagggACTCCGACAAATGGGCGAAT
Dmel	singed	singed_T7_1133F	taatacgactcactatagggATCTGTTTGCCACCTCGGAG	singed_T7_1444R	taatacgactcactatagggTTATGCAGATCCTGGTCGGC
Dmel	spz	spz_T7_553F	taatacgactcactatagggTTCTGCACGAATGTGGACGA	spz_T7_894R	taatacgactcactatagggGGTCTGCTGTGTGTAGTGCT
Dmel	spz	spz_T7_389F	taatacgactcactatagggAATCCGAACAGCCGATACCC	spz_T7_718R	taatacgactcactatagggACACCAGCTTCCTGATGCTC
Dmel	Ulp1	Ulp1_T7_186F	taatacgactcactatagggCAGCACGTTTCCAAGTTGGG	Ulp1_T7_617R	taatacgactcactatagggGCGACCTGGTTGTTTTGGTC
Dmel	Ulp1	Ulp1_T7_2991F	taatacgactcactatagggTAATGGCATCTCCGAGTCGC	Ulp1_T7_3512R	taatacgactcactatagggTCGTTGGCTTCCTTGACCTC
Dmel	cact	cact_T7_1F	taatacgactcactatagggGAGAACGCTGTGTGCATTTG	cact_T7_1R	taatacgactcactatagggGCTTCTCCAGGATGTTCTGC
Dmel	cact	cact_T7_318F	taatacgactcactatagggCCAAAAGGAACAGCCCGTTG	cact_T7_902R	taatacgactcactatagggGTGTTTCCATGACGATCGCG
KV	gp83	gp83_T7_200F	taatacgactcactatagggCCTCGAATCGTGCCAAATCG	gp83_T7_602R	taatacgactcactatagggTTGGAACAGAGACGGTCACG
KV	gp83	gp83_T7_583F	taatacgactcactatagggCGTGACCGTCTCTGTTCCAA	gp83_T7_882R	taatacgactcactatagggTTGGTCGGGTTTGAAAACGC
Plasmid	Firefly Luc	T7-Luc-F	taatacgactcactatagggAGATATGAAGAGATACGCCCTGGTT	T7-Luc-R	taatacgactcactatagggAGATAAAACCGGGAGGTAGATGAGA
Plasmid	GFP	T7-GFP-F	taatacgactcactatagggAGAAGCTGACCCTGAAGTTCATCTG	T7-GFP-R	taatacgactcactatagggAGAGGTGTTCTGCTGGTAGTGGTC

^aTemplates and primer names and sequences for cloning and dsRNA synthesis. Primer names include target amplicon, base coordinates of the target sequence that the 3′ end of the primer anneals to, and restriction enzyme or T7 binding site additions to the 5′ primer ends. For cloning, amplicons are shown, and for dsRNA synthesis, gene targets are shown. F and R, forward and reverse primers, respectively. Lowercase sequences are primer tails that include restriction enzyme cut-site, T7 polymerase promoter, and Kozak sequences; uppercase primer sequences are those that match the gene of interest.