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. 2019 Jan 18;476(2):179–191. doi: 10.1042/BCJ20180635

Figure 1. Purification of bacterially-expressed trypanosomatid and human PFKs.

Figure 1.

SDS–PAGE gels (4–20%) showing (A) trypanosomatid PFKs were produced at high purity but the removal of tags was only partially successful and (B) human PFK isoforms were produced at high purity but with the anomalous migration of PFK-L. Tagged enzymes were used for all experiments due to incomplete tag cleavage and reduction in enzyme activity after cleavage (data not shown), likely secondary to conditions required to remove tag. Ladder markers are in kDa.