Table 3. PFK-L has the lowest affinity for F16BP in the reverse reaction.

Mean average values with SEM values in brackets; n = 3.

	Vmax (µmol/min mg)	$K_{0.5}^{\mathbf{A}\mathbf{D}\mathbf{P}}$ (µM)	$K_{0.5}^{\mathbf{F}16\mathbf{B}\mathbf{P}}$ (µM)	h (ADP)	h (F16BP)
PFK-M	0.63 (0.05)	66.8 (13.4)	804 (66.1)	0.71 (0.22)	1.83 (0.23)
PFK-L	0.62 (0.01)	344.9 (36.4)	2100 (78.4)	1.39 (0.17)	2.49 (0.19)
PFK-P	0.22 (0)	ND	717.8 (58.1)	ND	1.35 (0.12)